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Kaedi, Zohreh, Narenjkar, Jamshai, Roghani, Mehrdad. (1397). The Effect of Quercetin on Learning and Memory Deficit, Lipid Peroxidation, and Cholinesterase Activeity Following Lipopolysaccharide in the Rat. , 7(1), 31-36. doi: 10.22070/jbcp.2019.4098.1110
Zohreh Kaedi; Jamshai Narenjkar; Mehrdad Roghani. "The Effect of Quercetin on Learning and Memory Deficit, Lipid Peroxidation, and Cholinesterase Activeity Following Lipopolysaccharide in the Rat". , 7, 1, 1397, 31-36. doi: 10.22070/jbcp.2019.4098.1110
Kaedi, Zohreh, Narenjkar, Jamshai, Roghani, Mehrdad. (1397). 'The Effect of Quercetin on Learning and Memory Deficit, Lipid Peroxidation, and Cholinesterase Activeity Following Lipopolysaccharide in the Rat', , 7(1), pp. 31-36. doi: 10.22070/jbcp.2019.4098.1110
Kaedi, Zohreh, Narenjkar, Jamshai, Roghani, Mehrdad. The Effect of Quercetin on Learning and Memory Deficit, Lipid Peroxidation, and Cholinesterase Activeity Following Lipopolysaccharide in the Rat. , 1397; 7(1): 31-36. doi: 10.22070/jbcp.2019.4098.1110

The Effect of Quercetin on Learning and Memory Deficit, Lipid Peroxidation, and Cholinesterase Activeity Following Lipopolysaccharide in the Rat

Journal of Basic and Clinical Pathophysiology
مقاله 5، دوره 7، شماره 1، خرداد 2019، صفحه 31-36    اصل مقاله (500.29 K)
نوع مقاله: Research Paper
شناسه دیجیتال (DOI): 10.22070/jbcp.2019.4098.1110
نویسندگان
Zohreh Kaedi1؛ Jamshai Narenjkar2؛ Mehrdad Roghani* 3
1School of Medicine, Shahed University, Tehran, Iran.
2Department of Pharmacology, School of Medicine, Shahed University, Tehran, Iran
3Neurophysiology Research Center, Shahed University, Tehran, Iran
چکیده
Background and Objective: Lipolysaccharide (LPS) is a large molecule isolated from bacteria such as the enterobacteriaceae family with a negative effect on memory and learning through disturbing the balance of free radicals and creating oxidative stress conditions. In this study, we evaluated  the effect of quercetin on oxidative stress and LPS-induced memory impairment in the rat.
Materials and Methods: Male rats (n=40) were randomly divided into 5 groups: control, control under treatment with quercetin at a dose of 50 mg/kg, LPS, and LPS groups treated with quercetin at doses of 10 or 50 mg/kg. For induction of inflammation, LPS dissolved in normal saline (500 μg/kg) was injected intraperitoneally. After one week, the passive avoidance behavior was tested in the shuttle box and hippocampal homogenate was prepared. Acetylcholinesterase (AChE) activity and lipid peroxidation (malondialdehyde, MDA) were measured using specific kits. Data were analyzed by SPSS software (version 16).
Results: Step-through latency (STL) in quercetin50-treated LPS group was significantly greater than control group (p<0.05). In addition, AChE activity and level of MDA was significantly lower in quercetin50-treated LPS group versus LPS group (p<0.05). Meanwhile, quercetin at a dose of 10 mg/kg did not have such a significant effect.
Conclusion: Quercetin at a dose of 50 mg/kg has a protective effect on learning and memory impairment due to LPS and part of its beneficial effect is mediated via attenuation of lipid peroxidation and AChE.
کلیدواژه‌ها
Lipolysaccharide؛ Quercetin؛ Neuroinflammation؛ Lipid peroxidation؛ Cholinesterase
مراجع
  1. Bettcher BM, Kramer JH. Longitudinal inflammation, cognitive decline, and Alzheimer's disease: a mini-review. Clinical Pharmacology and Therapeutics 2014;96(4):464-9.
  2. Metti AL, Cauley JA. How predictive of dementia are peripheral inflammatory markers in the elderly?. Neurodegenerative Disease Management 2012;2(6):609-22.
  3. Brites D, Fernandes A. Neuroinflammation and Depression: Microglia Activation, Extracellular Microvesicles and microRNA Dysregulation. Frontiers in Cellular Neuroscience 2015;9:476.
  4. Von Bernhardi R, Eugenin-von Bernhardi L, Eugenin J. Microglial cell dysregulation in brain aging and neurodegeneration. Frontiers in Cellular Neuroscience 2015;7:124.
  5. DeLegge MH, Smoke A. Neurodegeneration and inflammation. Nutrition in Clinical Practice 2008;23(1):35-41.
  6. Sun JS, Yang YJ, Zhang YZ, Huang W, Li ZS, Zhang Y. Minocycline attenuates pain by inhibiting spinal microglia activation in diabetic rats. Molecular Medicine Reports 2015;12(2):2677-82.
  7. Hsing CH, Hung SK, Chen YC, Wei TS, Sun DP, Wang JJ, et al. Histone Deacetylase Inhibitor Trichostatin A Ameliorated Endotoxin-Induced Neuroinflammation and Cognitive Dysfunction. Mediators Inflamm 2015;2015:163140.
  8. Jangra A, Sriram CS, Lahkar M. Lipopolysaccharide-Induced Behavioral Alterations Are Alleviated by Sodium Phenylbutyrate via Attenuation of Oxidative Stress and Neuroinflammatory Cascade. Inflammation 2016.
  9. Ming Z, Wotton CA, Appleton RT, Ching JC, Loewen ME, Sawicki G, et al. Systemic lipopolysaccharide-mediated alteration of cortical neuromodulation involves increases in monoamine oxidase-A and acetylcholinesterase activity. Journal of Neuroinflammation 2015;12:37.
  10. Russo M, Spagnuolo C, Tedesco I, Bilotto S, Russo GL. The flavonoid quercetin in disease prevention and therapy: facts and fancies. Biochemical Pharmacology 2012;83(1):6-15.
  11. Abdel-Diam MM, Samak DH, El-Sayed YS, Aleya L, Alarifi S, Alkahtani S. Curcumin and quercetin synergistically attenuate subacute diazinon-induced inflammation and oxidative neurohepatic damage, and acetylcholinesterase inhibition in albino rats. Environmental Science and Pollution Research International 2018.
  12. Halder S, Kar R, Chandra N, Nimesh A, Mehta AK, Bhattacharya SK, et al. Alteration in cognitive behaviour, brain antioxidant enzyme activity and their gene expression in F1 generation mice, following Cd exposure during the late gestation period: modulation by quercetin. Metabolic Brain Disease 2018;33(6):1935-43.
  13. Khan K, Najmi AK, Akhtar M. A Natural Phenolic Compound Quercetin Showed the Usefulness by Targeting Inflammatory, Oxidative Stress Markers and Augment 5-HT Levels in One of the Animal Models of Depression in Mice. Drug Research (Stuttg) 2018.
  14. Wang Q, Liu C. Protective effects of quercetin against brain injury in a rat model of lipopolysaccharide-induced fetal brain injury. International Journal of Developmental Neuroscience 2018;71:175-80.
  15. Du G, Zhao Z, Chen Y, Li Z, Tian Y, Liu Z, et al. Quercetin protects rat cortical neurons against traumatic brain injury. Molecular Medicine Reports 2018;17(6):7859-65.
  16. Bahar E, Kim JY, Yoon H. Quercetin Attenuates Manganese-Induced Neuroinflammation by Alleviating Oxidative Stress through Regulation of Apoptosis, iNOS/NF-kappaB and HO-1/Nrf2 Pathways. International Journal of Molecular Sciences 2017;18(9).
  17. Gao FJ, Zhang SH, Xu P, Yang BQ, Zhang R, Cheng Y, et al. Quercetin Declines Apoptosis, Ameliorates Mitochondrial Function and Improves Retinal Ganglion Cell Survival and Function in In Vivo Model of Glaucoma in Rat and Retinal Ganglion Cell Culture In Vitro. Frontiers in Molecular Neuroscience 2017;10:285.
  18. Li X, Wang H, Wen G, Li L, Gao Y, Zhuang Z, et al. Neuroprotection by quercetin via mitochondrial function adaptation in traumatic brain injury: PGC-1alpha pathway as a potential mechanism. Journal of Cellular and Molecular Medicine 2018;22(2):883-91.
  19. Dogan Z, Kocahan S, Erdemli E, Kose E, Yilmaz I, Ekincioglu Z, et al. Effect of chemotherapy exposure prior to pregnancy on fetal brain tissue and the potential protective role of quercetin. Cytotechnology 2015;67(6):1031-8.
  20. 20. Henry CJ, Huang Y, Wynne A, Hanke M, Himler J, Bailey MT, et al. Minocycline attenuates lipopolysaccharide (LPS)-induced neuroinflammation, sickness behavior, and anhedonia. Journal of Neuroinflammation 2008;5:15.
  21. Czerniawski J, Miyashita T, Lewandowski G, Guzowski JF. Systemic lipopolysaccharide administration impairs retrieval of context-object discrimination, but not spatial, memory: Evidence for selective disruption of specific hippocampus-dependent memory functions during acute neuroinflammation. Brain, Behavior, and Immunity 2015;44:159-66.
  22. Nasri S, Roghani M, Baluchnejadmojarad T, Rabani T, Balvardi M. Vascular mechanisms of cyanidin-3-glucoside response in streptozotocin-diabetic rats. Pathophysiology 2011;18(4):273-8.
  23. Ellman GL, Courtney KD, Andres V, Jr., Feather-Stone RM. A new and rapid colorimetric determination of acetylcholinesterase activity. Biochemical Pharmacology 1961;7:88-95.
  24. Barros DM, Pereira P, Medina JH, Izquierdo I. Modulation of working memory and of long- but not short-term memory by cholinergic mechanisms in the basolateral amygdala. Behavioral Pharmacology 2002;13(2):163-7.
  25. Ramirez MR, Izquierdo I, do Carmo Bassols Raseira M, Zuanazzi JA, Barros D, Henriques AT. Effect of lyophilised Vaccinium berries on memory, anxiety and locomotion in adult rats. Pharmacological Research 2005;52(6):457-62.
  26. Xu F, Proft J, Gibbs S, Winkfein B, Johnson JN, Syed N, et al. Quercetin targets cysteine string protein (CSPalpha) and impairs synaptic transmission. PLoS One 2010;5(6):e11045.
  27. Bai K, Xu W, Zhang J, Kou T, Niu Y, Wan X, et al. Assessment of Free Radical Scavenging Activity of Dimethylglycine Sodium Salt and Its Role in Providing Protection against Lipopolysaccharide-Induced Oxidative Stress in Mice. PLoS One 2016;11(5):e0155393.
  28. Sohanaki H, Baluchnejadmojarad T, Nikbakht F, Roghani M. Pelargonidin Improves Passive Avoidance Task Performance in a Rat Amyloid Beta25-35 Model of Alzheimer’s Disease Via Estrogen Receptor Independent Pathways. Acta Medica Iranica  2016:245-50.
  29. Mao XY, Cao DF, Li X, Yin JY, Wang ZB, Zhang Y, et al. Huperzine A ameliorates cognitive deficits in streptozotocin-induced diabetic rats. International Journal of Molecular Sciences 2014;15(5):7667-83.
  30. Liu ZH, Chen HG, Wu PF, Yao Q, Cheng HK, Yu W, et al. Flos Puerariae Extract Ameliorates Cognitive Impairment in Streptozotocin-Induced Diabetic Mice. Evidence-based Complementary and Alternative Medicine 2015;2015:873243.
  31. Tyagi E, Agrawal R, Nath C, Shukla R. Effect of melatonin on neuroinflammation and acetylcholinesterase activity induced by LPS in rat brain. European Journal of Pharmacology 2010;640(1-3):206-10.
  32. Silverman HA, Dancho M, Regnier-Golanov A, Nasim M, Ochani M, Olofsson PS, et al. Brain region-specific alterations in the gene expression of cytokines, immune cell markers and cholinergic system components during peripheral endotoxin-induced inflammation. Molecular Medicine 2014;20:601-11.
  33. Joshi R, Garabadu D, Teja GR, Krishnamurthy S. Silibinin ameliorates LPS-induced memory deficits in experimental animals. Neurobiology of Learning and Memory 2014;116:117-31.
  34. Abdel-Salam OM, Omara EA, Mohammed NA, Youness ER, Khadrawy YA, Sleem AA. Cerebrolysin attenuates cerebral and hepatic injury due to lipopolysaccharide in rats. Drug Discoveries & Therapeutics 2013;7(6):261-71.
  35. Lykhmus O, Mishra N, Koval L, Kalashnyk O, Gergalova G, Uspenska K, et al. Molecular Mechanisms Regulating LPS-Induced Inflammation in the Brain. Frontiers in Molecular Neuroscience 2016;9:19.

 

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