سامانه مدیریت نشریات علمی دانشگاه شاهد

Rezaee, Mohsen, Hajighasemi, Fatemeh. (1397). Sensitivity of Hematopoietic Malignant Cells to Peganum Harmala Seed Extract in Vitro. , 7(1), 21-26. doi: 10.22070/jbcp.2019.3982.1107
Mohsen Rezaee; Fatemeh Hajighasemi. "Sensitivity of Hematopoietic Malignant Cells to Peganum Harmala Seed Extract in Vitro". , 7, 1, 1397, 21-26. doi: 10.22070/jbcp.2019.3982.1107
Rezaee, Mohsen, Hajighasemi, Fatemeh. (1397). 'Sensitivity of Hematopoietic Malignant Cells to Peganum Harmala Seed Extract in Vitro', , 7(1), pp. 21-26. doi: 10.22070/jbcp.2019.3982.1107
Rezaee, Mohsen, Hajighasemi, Fatemeh. Sensitivity of Hematopoietic Malignant Cells to Peganum Harmala Seed Extract in Vitro. , 1397; 7(1): 21-26. doi: 10.22070/jbcp.2019.3982.1107

Sensitivity of Hematopoietic Malignant Cells to Peganum Harmala Seed Extract in Vitro

Journal of Basic and Clinical Pathophysiology
مقاله 1، دوره 7، شماره 1، خرداد 2019، صفحه 21-26    اصل مقاله (666.56 K)
نوع مقاله: Research Paper
شناسه دیجیتال (DOI): 10.22070/jbcp.2019.3982.1107
نویسندگان
Mohsen Rezaee؛ Fatemeh Hajighasemi*
Department of Immunology, Faculty of Medicine, Shahed University, Tehran, Iran.
چکیده
Background and Objective: Peganum harmala is a medicinal plant that has been used for treatment of numerous diseases including viral, bacterial and parasitic infections. Anti-proliferative and anti-tumor effects of Peganum harmala extracts and its derivatives have been reported. In the present study, cytotoxic effect of Peganum harmala seeds aquaous extract on leukemic U937 and Molt-4 cells was evaluated in vitro.
Materials and Methods: U937 and Molt-4 cells were cultured in RPMI with 10% FBS. Then, the cells at logarithmic growth phase were incubated with different concentrations of aquaous extract of Peganum harmala seeds (0.1-5 mg/ml) for 24, 48 and 72 hours. Then, viability and proliferative response of leukemic cell lines was evaluated by trypan blue dye exclusion (TB) and MTT assays, respectively.
Results: Peganum harmala aquaous extract has a cytotoxic effect on leukemic cells used in this study, dose and time-dependently. This cytotoxicity was shown at ≥ 0.5 mg/ml after 24 hours and at ≥ 0.1 mg/ml after 48 and 72 hours incubation time. The Peganum harmala seeds aqueous extract cytotoxicity at > 0.2 mg/ml concentration was significantly increased with time in this order: 72 h>48 h>24 h.
Conclusion: The leukemic cells used in this study showed sensitivity to Peganum harmala seeds aquaous extract dose and time dependently. This sensitivity significantly increased with time at > 0.2 mg/ml concentration. Peganum harmala seeds aquaous extract shows cytotoxicity for leukemic cells and might be a valuable natural candidate in development of innovative therapeutic procedures for leukemia and probably other cancers.
کلیدواژه‌ها
Peganum harmala؛ cytotoxicity؛ leukemia
مراجع
  1. Moradi MT, Karimi A, Rafieian-Kopaei M, Fotouhi F. In vitro antiviral effects of Peganum harmala seed extract and its total alkaloids against Influenza virus.  Microbial Pathogenesis 2017; 110:42-49.
  2. Valizadeh N, Valian F, Sadeghifard N, Karami S, Pakzad I, Kazemian H, Ghafourian S.The Role of Peganum harmala Ethanolic Extract and Type II Toxin Antitoxin System in Biofilm Formation. Drug Research (Stuttg) 2017; 67(7):385-387.
  3. Moazeni M, Saadaty Ardakani ZS, Saharkhiz MJ, Jalaei J, Khademolhoseini AA, Shams Esfand Abad S and Mootabi Alavi A. In vitro ovicidal activity of Peganum harmala seeds extract on the eggs of Fasciola hepatica. Journal of Parasitic Diseases 2017; 41(2):467-472.
  4. El Gendy MA, Somayaji V, El-Kadi AO. Peganum harmala L. is a candidate herbal plant for preventing dioxin mediated effects. Planta Medica 2010; 76(7):671-7.
  5. Daoud A, Song J, Xiao F, Shang J. B-9-3, a novel β-carboline derivative exhibits anti-cancer activity via induction of apoptosis and inhibition of cell migration in vitro. European Journal of Pharmacology 2014; 724:219-30.
  6. Oodi A, Norouzi H, Amirizadeh N, Nikougoftar M, Vafaie Z. Harmine, a Novel DNA Methyltransferase 1 Inhibitor in the Leukemia Cell Line. Indian Journal of Hematology and Blood Transfusion 2017; 33(4):509-515.
  7. Hamsa TP, Kuttan G. Harmine activates intrinsic and extrinsic pathways of apoptosis in B16F-10 melanoma. Chinese Medicine  2011; 6(1):11.
  8. Liu X, Li M, Tan S, Wang C, Fan S, Huang C. Harmine is an inflammatory inhibitor through the suppression of NF-κB signaling. Biochemical and Biophysical Research Communications 2017; 489(3):332-338.
  9. Hamsa TP, Kuttan G. Harmine inhibits tumour specific neo-vessel formation by regulating VEGF, MMP, TIMP and pro-inflammatory mediators both in vivo and in vitro. European Journal of Pharmacology 2010; 649:64–73.
  10.  Li C, Wang Y, Wang C, Yi X, Li M, He X. Anticancer activities of harmine by inducing a pro-death autophagy and apoptosis in human gastric cancer cells. Phytomedicine 2017; 28:10-18.
  11.  Shen J, Wang B, Zhang T, Zhu N, Wang Z, Jin J, He Y, Hu M.Suppression of Non-Small Cell Lung Cancer Growth and Metastasis by a Novel Small Molecular Activator of RECK. Cellular Physiology and Biochemistry 2018; 45(5):1807-1817.
  12. Yu XJ, Sun K, Tang XH, Zhou CJ, Sun H, Yan Z, Fang L, Wu HW, Xie YK2, Gu B1. Harmine combined with paclitaxel inhibits tumor proliferation and induces apoptosis through down-regulation of cyclooxygenase-2 expression in gastric cancer. Oncology Letters 2016; 12(2):983-988.
  13.  Ma Q, Chen W, Chen W. Anti-tumor angiogenesis effect of a new compound: B-9-3 through interference with VEGFR2 signaling. Tumor Biology 2016; 37(5):6107-16.
  14.  Zaker F, Oody A, Arjmand A. A study on the antitumoral and differentiation effects of Peganum harmala derivatives in combination with ATRA on leukaemic cells. Archives of Pharmacal Research 2007; 30(7):844-9.
  15.  Wang C, Zhang Z, Wang Y and He X. Cytotoxic Indole Alkaloids against Human Leukemia Cell Lines from the Toxic Plant Peganum harmala. Toxins 2015; 7:4507-4518.
  16.  Hashemi Sheikh Shabani S, Seyed Hasan Tehrani S, Rabiei Z, Tahmasebi Enferadi S, Vannozzi GP. Peganum harmala L.'s anti-growth effect on a breast cancer cell line. Biotechnology Reports (Amst) 2015; 8:138-143.
  17.  Chabir N, Ibrahim H, Romdhane H, Valentin A, Moukarzel B, Mars M, Bouajila J. Seeds of Peganum Harmala L. chemical analysis, antimalarial and antioxidant activities, and cytotoxicity against human breast cancer cells. Medicinal Chemistry 2014; 11(1):94-101.
  18.  Gao J, Zhu H, Wan H, Zou X, Ma X, Gao G: Harmine suppresses the proliferation and migration of human ovarian cancer cells through inhibiting ERK/CREB pathway. Oncology Reports 2017; 38(5):2927-2934. 
  19.  Shen J, Wang B, Zhang T, Zhu N, Wang Z, Jin J, He Y, Hu M. Suppression of Non-Small Cell Lung Cancer Growth and Metastasis   by Novel Small Molecular Activator of RECK. Cellular Physiology and Biochemistry 2018; 45(5):1807-1817. 
  20. Rezaei M, Nasri S, Roughani M, Niknami Z, Ziai SA. Peganum Harmala L. Extract Reduces Oxidative Stress and Improves Symptoms in 6-Hydroxydopamine Induced Parkinson's diseases in Rats. Iranian Journal of pharmaceutical research 2016; 15(1): 275-81.
  21.  Moldeus P, Hogberg J, Orrenius S. Isolation and use of liver cells. Methods in Enzymology 1978; 52:60-71.
  22.  Mosmann T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. Journal of Immunological Methods 1983; 65(1-2):55-63.
  23.  Forouzandeh F, Salimi S, Naghsh N, Zamani N, Jahani S. Evaluation of anti-cancer effect of Peganum harmala L hydroalcholic extract on human cervical carcinoma epithelial cell line. Journal of Shahrekord University of Medical Sciences 2014; 16(4):1-8. (Persian).
  24.  Shirazi FH, Ahmadi N, Kamalinejad M. Evaluation of northern Iran Mentha pulegium L. cytotoxicity. Daru 2004; 2(3): 106-110.
  25.  Hajighasemi F, Tajik S. Assessment of Cytotoxicity of Dimethyl Sulfoxide in Human
  26. Hematopoietic Tumor Cell Lines. Iranian Journal of Blood and Cancer 2017; 9(2): 48-53.
  27.  Mahajna S, Azab M, Zaid H, Abo Farich B. In vitro Evaluations of Cytotoxicity and
  28. Anti-inflammatory Effects of Peganum harmala Seed Extracts in THP-1-derived Macrophages. European Journal of Medicinal Plants 2015; 5(2): 165-175.
  29.  Gao P, Tao N, Ma Q, Fan WX, Ni C, Wang H, Qin ZH. DH332, a synthetic β-carboline alkaloid, inhibits B cell lymphoma growth by activation of the caspase family. Asian Pacific Journal of Cancer Prevention 2014;15(9):3901-6.
  30.  Li SP, Wang YW, Qi SL, Zhang YP, Deng G, Ding WZ, Ma C, et al. Analogous β-Carboline Alkaloids Harmaline and Harmine Ameliorate Scopolamine-Induced Cognition Dysfunction by Attenuating Acetylcholinesterase Activity, Oxidative Stress, and Inflammation in Mice. Frontiers in Pharmacology 2018; 9:346.
  31. Wei Y, Ma T, Wang H, Xing J, Wang Y, Gu Z, et al. Extracts of compound Muniziqi granule suppressed uterus contraction and ameliorated oxytocin-induced primary dysmenorrhea. Journal of Ethnopharmacology 2018; 223:33-40.
  32. Boniakowski AE, Kimball AS, Joshi A, Schaller M, Davis FM, denDekker A, et al. Murine macrophage chemokine receptor CCR2 plays a crucial role in macrophage recruitment and regulated inflammation in wound healing. European Journal of Immunology 2018; 48(9):1445-1455.
  33. Andersen BL, Goyal NG, Weiss DM, Westbrook TD, Maddocks KJ, Byrd JC, Johnson AJ. Cells, cytokines, chemokines, and cancer stress: A biobehavioral study of patients with chronic lymphocytic leukemia. Cancer 2018; 124(15):3240-3248. 
  34. Cheong DHJ, Arfuso F, Sethi G, Wang L, Hui KM, Kumar AP, Tran T. Molecular targets and anti-cancer potential of escin. Cancer Letters 2018; 422:1-8.
آمار
تعداد مشاهده مقاله: 554
تعداد دریافت فایل اصل مقاله: 449