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Raoufi, Safoura, Baluchnejadmojarad, Tourandokht. (1396). Salvianolic acid B improves insulin secretion from interleukin 1β-treated rat pancreatic islets: The role of PI3K-Akt signaling. , 6(1), 49-55. doi: 10.22070/jbcp.2017.2863.1089
Safoura Raoufi; Tourandokht Baluchnejadmojarad. "Salvianolic acid B improves insulin secretion from interleukin 1β-treated rat pancreatic islets: The role of PI3K-Akt signaling". , 6, 1, 1396, 49-55. doi: 10.22070/jbcp.2017.2863.1089
Raoufi, Safoura, Baluchnejadmojarad, Tourandokht. (1396). 'Salvianolic acid B improves insulin secretion from interleukin 1β-treated rat pancreatic islets: The role of PI3K-Akt signaling', , 6(1), pp. 49-55. doi: 10.22070/jbcp.2017.2863.1089
Raoufi, Safoura, Baluchnejadmojarad, Tourandokht. Salvianolic acid B improves insulin secretion from interleukin 1β-treated rat pancreatic islets: The role of PI3K-Akt signaling. , 1396; 6(1): 49-55. doi: 10.22070/jbcp.2017.2863.1089

Salvianolic acid B improves insulin secretion from interleukin 1β-treated rat pancreatic islets: The role of PI3K-Akt signaling

Journal of Basic and Clinical Pathophysiology
مقاله 8، دوره 6، شماره 1، اردیبهشت 2018، صفحه 49-55    اصل مقاله (242.71 K)
نوع مقاله: Research Paper
شناسه دیجیتال (DOI): 10.22070/jbcp.2017.2863.1089
نویسندگان
Safoura Raoufi* 1؛ Tourandokht Baluchnejadmojarad2
1Neurophysiology Research Center, Hamadan University of Medical Sciences, Hamadan, Iran
2Department of Physiology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.
چکیده
Background and Objective: Oxidative stress induced by proinflammatory cytokines such as IL-1β plays a major role in β-cell destruction in diabetes type 1. Salvianolic acid B (Sal B) is a polyphenolic compound with antioxidant and protective effects. Thus, objective of this study was to assess the protection exerted by Sal B on isolated rat islets exposed to IL-1β and to investigate an underlying mechanism in vitro.
Materials and Methods: Isolation of pancreatic islets was done by using the collagenase digestion method. Isolated rat islets were divided into 6 groups including: 1. control, 2. interleukin-1β treated, 3 and 4. interleukin-1β treated+ Sal B, 5 and 6. interleukin-1β treated+ Sal B+ PKB and PI3K inhibitors. Interleukin-1β (1 U/ml) was used to induce cytotoxicity after pretreatment with two doses of Sal B (50 μM and 100 μM) and application of each inhibitors was before Sal B.
Results: IL-1β significantly decreased insulin secretion from isolated islets. Pretreatment with Sal B ameliorated the effect of IL-1β on glucose stimulated insulin secretion in a concentration dependent manner. Inhibitors of PKB and PI3K both abolished these improving effect of Sal B.
Conclusion: Sal B that has antioxidant, anti inflammatory and anti apoptotic properties, provided resistance to pancreatic β-cell dysfunction from cytokine in part via PI3K/Akt pathway. The findings represent that it is a promising agent for prevention of β-cell dysfunction in type 1 diabetes.
کلیدواژه‌ها
Salvianolic acid B؛ Insulin؛ Pancreatic islet؛ Pancreatic β-cells؛ Interleukin 1β؛ PI3K-Akt
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