Salvianolic acid B improves insulin secretion from interleukin 1β-treated rat pancreatic islets: The role of PI3K-Akt signaling | ||
| Journal of Basic and Clinical Pathophysiology | ||
| مقاله 8، دوره 6، شماره 1، 2018، صفحه 49-55 اصل مقاله (242.71 K) | ||
| نوع مقاله: Research Paper | ||
| شناسه دیجیتال (DOI): 10.22070/jbcp.2017.2863.1089 | ||
| نویسندگان | ||
| Safoura Raoufi* 1؛ Tourandokht Baluchnejadmojarad2 | ||
| 1Neurophysiology Research Center, Hamadan University of Medical Sciences, Hamadan, Iran | ||
| 2Department of Physiology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran. | ||
| چکیده | ||
| Background and Objective: Oxidative stress induced by proinflammatory cytokines such as IL-1β plays a major role in β-cell destruction in diabetes type 1. Salvianolic acid B (Sal B) is a polyphenolic compound with antioxidant and protective effects. Thus, objective of this study was to assess the protection exerted by Sal B on isolated rat islets exposed to IL-1β and to investigate an underlying mechanism in vitro. Materials and Methods: Isolation of pancreatic islets was done by using the collagenase digestion method. Isolated rat islets were divided into 6 groups including: 1. control, 2. interleukin-1β treated, 3 and 4. interleukin-1β treated+ Sal B, 5 and 6. interleukin-1β treated+ Sal B+ PKB and PI3K inhibitors. Interleukin-1β (1 U/ml) was used to induce cytotoxicity after pretreatment with two doses of Sal B (50 μM and 100 μM) and application of each inhibitors was before Sal B. Results: IL-1β significantly decreased insulin secretion from isolated islets. Pretreatment with Sal B ameliorated the effect of IL-1β on glucose stimulated insulin secretion in a concentration dependent manner. Inhibitors of PKB and PI3K both abolished these improving effect of Sal B. Conclusion: Sal B that has antioxidant, anti inflammatory and anti apoptotic properties, provided resistance to pancreatic β-cell dysfunction from cytokine in part via PI3K/Akt pathway. The findings represent that it is a promising agent for prevention of β-cell dysfunction in type 1 diabetes. | ||
| کلیدواژهها | ||
| Salvianolic acid B؛ Insulin؛ Pancreatic islet؛ Pancreatic β-cells؛ Interleukin 1β؛ PI3K-Akt | ||
| مراجع | ||
|
| ||
|
آمار تعداد مشاهده مقاله: 1,011 تعداد دریافت فایل اصل مقاله: 693 |
||
| تعداد نشریات | 18 |
| تعداد شمارهها | 484 |
| تعداد مقالات | 4,757 |
| تعداد مشاهده مقاله | 6,035,330 |
| تعداد دریافت فایل اصل مقاله | 4,962,972 |