سامانه مدیریت نشریات علمی دانشگاه شاهد

Mirabi, HamidReza, Azizzadeh-Delshad, Alireza, Roghani, Mehrdad. (1403). Protective effect of Nigella sativa phytochemical thymoquinone in a mouse model of brain injury induced by potassium dichromate. , 12(2), 29-37. doi: 10.22070/jbcp.2024.19560.1183
HamidReza Mirabi; Alireza Azizzadeh-Delshad; Mehrdad Roghani. "Protective effect of Nigella sativa phytochemical thymoquinone in a mouse model of brain injury induced by potassium dichromate". , 12, 2, 1403, 29-37. doi: 10.22070/jbcp.2024.19560.1183
Mirabi, HamidReza, Azizzadeh-Delshad, Alireza, Roghani, Mehrdad. (1403). 'Protective effect of Nigella sativa phytochemical thymoquinone in a mouse model of brain injury induced by potassium dichromate', , 12(2), pp. 29-37. doi: 10.22070/jbcp.2024.19560.1183
Mirabi, HamidReza, Azizzadeh-Delshad, Alireza, Roghani, Mehrdad. Protective effect of Nigella sativa phytochemical thymoquinone in a mouse model of brain injury induced by potassium dichromate. , 1403; 12(2): 29-37. doi: 10.22070/jbcp.2024.19560.1183

Protective effect of Nigella sativa phytochemical thymoquinone in a mouse model of brain injury induced by potassium dichromate

Journal of Basic and Clinical Pathophysiology
دوره 12، شماره 2، دی 2024، صفحه 29-37    اصل مقاله (629.73 K)
نوع مقاله: Research Paper
شناسه دیجیتال (DOI): 10.22070/jbcp.2024.19560.1183
نویسندگان
HamidReza Mirabi1؛ Alireza Azizzadeh-Delshad2؛ Mehrdad Roghani* 3
1School of Medicine, Shahed University, Tehran, Iran
2Department of Anatomy, School of Medicine, Shahed University, Tehran, Iran.
3Neurophysiology Research Center, Shahed University, Tehran, Iran
چکیده
Background and Objective: Potassium dichromate, which is a form of hexavalent chromium, has been demonstrated to induce toxicity associated with oxidative stress in humans and animals. Thymoquinone (TQ) isolated from Nigella sativa has a wide spectrum of activities such as antioxidant, anti-inflammatory and anticancer effects. This study aimed to evaluate the protective effect of thymoquinone in an animal model of brain damage caused by potassium dichromate in the mouse.
Materials and Methods: In this study, mice were divided into 5 groups: control, control treated with 5 mg/kg thymoquinone, potassium dichromate, and potassium dichromate treated with 1 or 5 mg/kg thymoquinone. To induce neuronal injury, 0.5 mg/kg potassium dichromate was intranasally and daily administrated for two months. Thymoquinone was given orally and daily for seven weeks. After two months, activity of catalase (CAT), level of malondialdehyde (MDA), glutathione (GSH) and nitrite (Nit), and number of neurons in the parietal cortex were evaluated. Data analysis was conducted using one-way ANOVA and Tukey post-test with p<0.05 as significant.
Results: Potassium dichromate significantly lowered activity of CAT and elevated levels of MDA and Nit, reduced GSH level and decreased neuronal density in the parietal cortex. Treatment with 5 mg/kg thymoquinone significantly increased activity of CAT and reduced MDA level, elevated level of GSH and also prevented reduction of neuronal density in the parietal cortex.
Conclusion: Thymoquinone administration can lower oxidative stress and brain injury following potassium dichromate.
کلیدواژه‌ها
Potassium dichromate؛ Thymoquinone؛ Oxidative stress؛ Parietal cortex
مراجع
  1. Ma Z, He Z, Li Z, Gong R, Hui J, Weng W, et al. Traumatic brain injury in elderly population: A global systematic review and meta-analysis of in-hospital mortality and risk factors among 2.22 million individuals. Ageing Research Reviews. 2024;99:102376.
  2. Chevignard M, Câmara-Costa H, Dellatolas G. Predicting and improving outcome in severe pediatric traumatic brain injury. Expert Review of Neurotherapeutics. 2024:1-21.
  3. Sharma N, Sodhi KK, Kumar M, Singh DK. Heavy metal pollution: Insights into chromium eco-toxicity and recent advancement in its remediation. Environmental Nanotechnology, Monitoring & Management. 2021;15:100388.
  4. Tchounwou PB, Yedjou CG, Patlolla AK, Sutton DJ. Heavy metal toxicity and the environment. Experientia Supplementum. 2012;101:133-64.
  5. Crocetto F, Risolo R, Colapietro R, Bellavita R, Barone B, Ballini A, et al. Heavy Metal Pollution and Male Fertility: An Overview on Adverse Biological Effects and Socio-Economic Implications. Endocrine, Metabolic & Immune Disorders - Drug Targets. 2023;23(2):129-46.
  6. Azeez NA, Dash SS, Gummadi SN, Deepa VS. Nano-remediation of toxic heavy metal contamination: Hexavalent chromium [Cr(VI)]. Chemosphere. 2021;266:129204.
  7. Houldsworth A. Role of oxidative stress in neurodegenerative disorders: a review of reactive oxygen species and prevention by antioxidants. Brain Communications. 2024;6(1):fcad356.
  8. Kmail A, Said O, Saad B. How Thymoquinone from Nigella sativa Accelerates Wound Healing through Multiple Mechanisms and Targets. Current Issues in Molecular Biology. 2023;45(11):9039-59.
  9. Kurowska N, Madej M, Strzalka-Mrozik B. Thymoquinone: A Promising Therapeutic Agent for the Treatment of Colorectal Cancer. Current Issues in Molecular Biology. 2023;46(1):121-39.
  10. Saadat M, Dahmardeh N, Sheikhbahaei F, Mokhtari T. Therapeutic potential of thymoquinone and its nanoformulations in neuropsychological disorders: a comprehensive review on molecular mechanisms in preclinical studies. Naunyn-Schmiedeberg's Archives of Pharmacology. 2024;397(6):3541-64.
  11. Shaukat A, Zaidi A, Anwar H, Kizilbash N. Mechanism of the antidiabetic action of Nigella sativa and Thymoquinone: a review. Frontiers in Nutrition. 2023;10:1126272.
  12. Zhang H, Liu Y, Dong Y, Li G, Wang S. Thymoquinone: An Effective Natural Compound for Kidney Protection. The American Journal of Chinese Medicine. 2024;52(3):775-97.
  13. Patlolla AK, Barnes C, Hackett D, Tchounwou PB. Potassium dichromate induced cytotoxicity, genotoxicity and oxidative stress in human liver carcinoma (HepG2) cells. International Journal of Environmental Research and Public Health. 2009;6(2):643-53.
  14. Wise JP, Jr., Young JL, Cai J, Cai L. Current understanding of hexavalent chromium [Cr(VI)] neurotoxicity and new perspectives. Environment International. 2022;158:106877.
  15. Iyer M, Anand U, Thiruvenkataswamy S, Babu HWS, Narayanasamy A, Prajapati VK, et al. A review of chromium (Cr) epigenetic toxicity and health hazards. Science of The Total Environment. 2023;882:163483.
  16. Dashti A, Soodi M, Amani N. Evaluation of Cr (VI) induced Neurotoxicity and Oxidative Stress in PC12 Cells. Pathobiology Research. 2015;18(1):55-65.
  17. Jelinek M, Jurajda M, Duris K. Oxidative Stress in the Brain: Basic Concepts and Treatment Strategies in Stroke. Antioxidants (Basel). 2021;10(12).
  18. Salim S. Oxidative Stress and the Central Nervous System. Journal of Pharmacology and Experimental Therapeutics. 2017;360(1):201-5.
  19. Lushchak OV, Kubrak OI, Nykorak MZ, Storey KB, Lushchak VI. The effect of potassium dichromate on free radical processes in goldfish: Possible protective role of glutathione. Aquatic Toxicology. 2008;87(2):108-14.
  20. Bashandy SAE, Ebaid H, Al-Tamimi J, Ahmed-Farid OA, Omara EA, Alhazza IM. Melatonin Alleviated Potassium Dichromate-Induced Oxidative Stress and Reprotoxicity in Male Rats. BioMed Research International. 2021;2021:3565360.
  21. Aycan I, Tüfek A, Tokgöz O, Evliyaoğlu O, Fırat U, Kavak G, et al. Thymoquinone treatment against acetaminophen-induced hepatotoxicity in rats. International Journal of Surgery. 2014;12(3):213-8.
  22. Tian F, Liu R, Fan C, Sun Y, Huang X, Nie Z, et al. Effects of Thymoquinone on Small-Molecule Metabolites in a Rat Model of Cerebral Ischemia Reperfusion Injury Assessed using MALDI-MSI. Metabolites. 2020;10(1).
  23. Abbas F, Eladl MA, El-Sherbiny M, Abozied N, Nabil A, Mahmoud SM, et al. Celastrol and thymoquinone alleviate aluminum chloride-induced neurotoxicity: Behavioral psychomotor performance, neurotransmitter level, oxidative-inflammatory markers, and BDNF expression in rat brain. Biomedicine & Pharmacotherapy. 2022;151:113072.
  24. Alrafiah A. Thymoquinone Protects Neurons in the Cerebellum of Rats through Mitigating Oxidative Stress and Inflammation Following High-Fat Diet Supplementation. Biomolecules. 2021;11(2).
  25. Hafez MH, Ez Elarab SM, Tohamy HG, El-Far AH. Thymoquinone attenuates diabetes-induced hepatic damage in rat via regulation of oxidative/nitrosative stress, apoptosis, and inflammatory cascade with molecular docking approach. Scientific Reports. 2024;14(1):13016.
  26. Elsherbiny NM, Maysarah NM, El-Sherbiny M, Al-Gayyar MM. Renal protective effects of thymoquinone against sodium nitrite-induced chronic toxicity in rats: Impact on inflammation and apoptosis. Life Sciences. 2017;180:1-8.
  27. Hamdan AM, Al-Gayyar MM, Shams MEE, Alshaman US, Prabahar K, Bagalagel A, et al. Thymoquinone therapy remediates elevated brain tissue inflammatory mediators induced by chronic administration of food preservatives. Scientific Reports. 2019;9(1):7026.
  28. Parvin Salehi, Sima Nasri, Mehrdad Roghani, Uranus Poordahandeh, Tourandokht Baluchnejadmojarad. The effect of thymoquinone on short-term spatial memory, passive avoidance learning and memory of diabetic rats and the involvement of hippocampal oxidative stress. Pajoohande. 2012;17(5):219-27.
  29. Nazir S, Farooq RK, Nasir S, Hanif R, Javed A. Therapeutic effect of Thymoquinone on behavioural response to UCMS and neuroinflammation in hippocampus and amygdala in BALB/c mice model. Psychopharmacology (Berl). 2022;239(1):47-58.
آمار
تعداد مشاهده مقاله: 177
تعداد دریافت فایل اصل مقاله: 172