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Kakavand, Shima, Karami, Manizheh. (1403). Effect of nitric oxide synthase inhibition on ovarian cystogenesis induced by morphine injection into ventro-medial nucleus of hypothalamus (VMH) of female Wistar rats. , 12(2), 22-28. doi: 10.22070/jbcp.2024.19455.1181
Shima Kakavand; Manizheh Karami. "Effect of nitric oxide synthase inhibition on ovarian cystogenesis induced by morphine injection into ventro-medial nucleus of hypothalamus (VMH) of female Wistar rats". , 12, 2, 1403, 22-28. doi: 10.22070/jbcp.2024.19455.1181
Kakavand, Shima, Karami, Manizheh. (1403). 'Effect of nitric oxide synthase inhibition on ovarian cystogenesis induced by morphine injection into ventro-medial nucleus of hypothalamus (VMH) of female Wistar rats', , 12(2), pp. 22-28. doi: 10.22070/jbcp.2024.19455.1181
Kakavand, Shima, Karami, Manizheh. Effect of nitric oxide synthase inhibition on ovarian cystogenesis induced by morphine injection into ventro-medial nucleus of hypothalamus (VMH) of female Wistar rats. , 1403; 12(2): 22-28. doi: 10.22070/jbcp.2024.19455.1181

Effect of nitric oxide synthase inhibition on ovarian cystogenesis induced by morphine injection into ventro-medial nucleus of hypothalamus (VMH) of female Wistar rats

Journal of Basic and Clinical Pathophysiology
دوره 12، شماره 2، دی 2024، صفحه 22-28    اصل مقاله (579.79 K)
نوع مقاله: Research Paper
شناسه دیجیتال (DOI): 10.22070/jbcp.2024.19455.1181
نویسندگان
Shima Kakavand1؛ Manizheh Karami* 2
1Department of Biology, Faculty of Basic Sciences, Shahed University, Tehran, Iran
2Neurophysiology Research Center, Shahed University, Tehran, Iran
چکیده
Background and Objective: Polycystic ovary (PCO) causes infertility and morphine use has been shown to induce this disorder. Microinjection of L-NAME, a competitive inhibitor of nitric oxide synthase (NOS) into the ventro-medial hypothalamus (VMH) may interfere with this effect as our aim
Materials and Methods: Female Wistar rats with a weight range of 200 to 250 g received morphine (0.001-0.4 µg/rat) intra-VMH with AP coordinates= -1.96. L-NAME (0.1-0.4 μg/rat, intra-VMH) was used alone or as a pre-injection to the effective dose of morphine. L-arginine (0.1-0.4 μg/rat, intra-VMH) was also injected alone or cumulatively with the inhibitor to elucidate the mechanism. Control group received saline (1 µL/rat), intra-VMH. Finally, the animals were anesthetized with ketamine and xylazine and the ovaries and uterus were dissected and examined pathologically. All data were analyzed using ANOVA (α= 0.05).
Results: Ovaries of morphine-treated rats showed a polycystic appearance, but the number of cysts in rats receiving L-NAME, intra-VMH, was significantly reduced. L-NAME/L-arginine intervention in the presence of morphine also had a reducing effect on cyst formation.
Conclusion: Induction of PCO by morphine injection in VMH may be involved in opioid receptors signaling and NOS enzyme activation, which is slowed by L-NAME intervention.
کلیدواژه‌ها
Morphine؛ L-NAME؛ L-Arginine؛ Ventro-medial hypothalamus؛ Polycystic ovary
مراجع
  1. Kalem MN, Kalem Z, Gurgan T. Effect of metformin and oral contraceptives on polycystic ovary syndrome and IVF cycles. Journal of Endocrinological Investigation. 2017:1-8.‏
  2. Malik-Aslam A, Reaney MD, Speight J. The suitability of polycystic ovary syndrome-specific questionnaires for measuring the impact of PCOS on quality of life in clinical trials. Value in Health. 2010;13:440-446.‏
  3. Lopes IMRS, Baracat MCP, Simoes M, Simoes RS, Baracat EC, Soares Jr JM. Endometrium in women with polycystic ovary syndrome during the window of implantation. Revista da Associação Médica Brasileira (English Edition). 2011;57:688-695.‏
  4. Singh KB. Rat models of polycystic ovary syndrome. Sourcebook of models for biomedical research. Humana Press, 2008, p: 405-410.‏
  5. Calogero AE. Understanding polycystic ovarian syndrome pathogenesis: an updated of its genetic aspects. Journal of Endocrinological Investigation. 2011;34:630-644.‏
  6. Goswami PK, Anubha K, Ogale S. Natural remedies for polycystic ovarian syndrome (PCOS): a review. International Journal of Pharmaceutical and Phytopharmacological Research. 2017;1:396-402.‏
  7. Indhavivadhana S, Rattanachaiyanont M, Wongwananuruk T, Techatraisak K, Tanmahasamut P, Dangrat C. Brief communication (Original). Hyperandrogenemia is associated with thin endometrium in reproductive-aged Thai women with polycystic ovary syndrome. Asian Biomedicine. 2013;7:545-551.‏
  8. Rosselli M, Keller PJ, Dubey RK. Role of nitric oxide in the biology, physiology and pathophysiology of reproduction. Human Reproduction Update. 1998;4:3-24.‏
  9. Khan FA, Chenier TS, Murrant CL, Foster RA, Hewson J, Scholtz EL. Dose-dependent inhibition of uterine contractility by nitric oxide: A potential mechanism underlying persistent breeding-induced endometritis in the mare. Theriogenology. 2017;90:59-64.
  10. Hoang HH, Padgham SV, Meininger CJ. L-arginine, tetrahydrobiopterin, nitric oxide and diabetes. Current Opinion in Clinical Nutrition & Metabolic Care. 2013;16:76-82.
  11. Zamberlam G, Portela V, de Oliveira JFC, Gonçalves PBD, Price CA. Regulation of inducible nitric oxide synthase expression in bovine ovarian granulosa cells." Molecular and Cellular Endocrinology. 2011;335:189-194.
  12. Dubey PK, Sharma GT. Nitric Oxide and Ovarian Folliculogenesis: A Possible Role in Follicular Atresia-A Review. 2016.
  13. Brook K, Bennett J, Desai SP. The Chemical History of Morphine: An 8000-year journey, from resin to de-novo synthesis. Journal of Anesthesia History. 2017.
  14. ‏Buss T, Leppert W. Opioid-induced endocrinopathy in cancer patients: an underestimated clinical problem. Advances in Therapy. 2014;31:153-167.
  15. Wang X, Stocco DM. The decline in testosterone biosynthesis during male aging: a consequence of multiple alterations. Molecular and Cellular Endocrinology. 2005;238:1-7.
  16. Rachdaoui N, Sarkar DK. Pathophysiology of the Effects of Alcohol Abuse on the Endocrine System. Alcohol Research. 2017;38:E1.
  17. Asimakopoulos B. Hypothalamus-Pituitary-Gonadal axis: It is time for revision. Human Genetics & Embryology. 2012;1:e106.
  18. Emanuele MA, Wezeman F, Emanuele NV. Alcohol's effects on female reproductive function. Alcohol Research and Health. 2002;26:274-281.
  19. Dixit VDeep, Parvizi N. Nitric oxide and the control of reproduction. Animal Reproduction Science. 2001;65:1-16.
  20. Calka J. The role of nitric oxide in the hypothalamic control of LHRH and oxytocin release, sexual behavior and aging of the LHRH and oxytocin neurons. Folia Histochemica et Cytobiologica. 2006;44:3.
  21. Dong Y-L. Gangula PRR, Fang L, Yallampalli C. Nitric oxide reverses prostaglandin-induced inhibition in ovarian progesterone secretion in rats. Human Reproduction. 1999;14:27-32.
  22. Quiñones-Jenab V, Jenab S, Ogawa S, Inturrisi C, Pfaff DW. Estrogen regulation of μ-opioid receptor mRNA in the forebrain of female rats. Molecular Brain Research. 1997;47:134-138.‏
  1. Mathews D, Edwards DA. Involvement of the ventromedial and anterior hypothalamic nuclei in the hormonal induction of receptivity in the female rat. Physiology & Behavior. 1977;19:319-326.‏
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