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Mansouri, Mobina, Sadeghpour, Masoomeh, Rasooli, Iraj. (1401). Immunogenicity of the combination of periplasmic proteins BauA, Oma87, and Bap from Acinetobacter baumannii in a murine sepsis model. , 10(2), 1-8. doi: 10.22070/jbcp.2022.16939.1162
Mobina Mansouri; Masoomeh Sadeghpour; Iraj Rasooli. "Immunogenicity of the combination of periplasmic proteins BauA, Oma87, and Bap from Acinetobacter baumannii in a murine sepsis model". , 10, 2, 1401, 1-8. doi: 10.22070/jbcp.2022.16939.1162
Mansouri, Mobina, Sadeghpour, Masoomeh, Rasooli, Iraj. (1401). 'Immunogenicity of the combination of periplasmic proteins BauA, Oma87, and Bap from Acinetobacter baumannii in a murine sepsis model', , 10(2), pp. 1-8. doi: 10.22070/jbcp.2022.16939.1162
Mansouri, Mobina, Sadeghpour, Masoomeh, Rasooli, Iraj. Immunogenicity of the combination of periplasmic proteins BauA, Oma87, and Bap from Acinetobacter baumannii in a murine sepsis model. , 1401; 10(2): 1-8. doi: 10.22070/jbcp.2022.16939.1162

Immunogenicity of the combination of periplasmic proteins BauA, Oma87, and Bap from Acinetobacter baumannii in a murine sepsis model

Journal of Basic and Clinical Pathophysiology
دوره 10، شماره 2، اسفند 2022، صفحه 1-8    اصل مقاله (525.39 K)
نوع مقاله: Research Paper
شناسه دیجیتال (DOI): 10.22070/jbcp.2022.16939.1162
نویسندگان
Mobina Mansouri1؛ Masoomeh Sadeghpour1؛ Iraj Rasooli* 2
1Department of Biology, Shahed University, Tehran, Iran
2Molecular Microbiology Research Center, Shahed University, Tehran, Iran of Biology, Shahed University
چکیده
Background and Objective: Acinetobacter baumannii is a serious challenge to the healthcare system as a multi-drug-resistant bacterium. BauA is a siderophore of A. baumannii. Oma87 has been stated as an immunogenic outer membrane protein per reverse vaccinology. Biofilm-associated protein (Bap) plays an indispensable role in biofilm formation by this pathogen and makes it resistant to a wide range of antibiotics and lethal conditions. The persistence and colonization of A. baumannii can be prevented by raising antibodies against BauA, Oma87, and Bap.
Materials and Methods: The recombinant proteins BauA, Oma87, and Bap were expressed and purified and injected subcutaneously in single and/or in combination forms to BALB/c mice and then they were challenged intraperitoneally with a lethal dose of 50% A. baumannii ATCC19606. The serum of combined mice was injected intravenously through the tail vein to create passive immunity in non-immune healthy mice. The mice's spleen, liver, and lung tissues were examined to check the bacterial load.
Results: Oma87 and Bap in combination with BauA in the mouse model brought about significant immunity. Antibodies produced in mice successfully detected and bound the combination of antigens in the mouse model. After immunizing and challenging mice with Acinetobacter baumannii ATCC19606 admixed with mucin 100%, the survival rate was monitored. Passive immunization using the sera of mice immunized against Oma87 and BauA as well as BauA and Bap yielded 85.7% survival.
Conclusion: The combination of BauA with Oma87 and Bap brought about higher protectivity against A. baumannii infection than their individual administration.
کلیدواژه‌ها
A. baumannii؛ BauA؛ Bap؛ Oma87؛ Immunogen
مراجع
  1. Oyejobi GK, Olaniyan SO, Yusuf N-A, Ojewande DA, Awopetu MJ, Oyeniran GO, et al. Acinetobacter Baumannii: More Ways to Die. Microbiological Research 2022 ;261:127069. doi: 10.1016/j.micres.2022.127069.
  2. Morris FC, Dexter C, Kostoulias X, Uddin MI, Peleg AY. The mechanisms of disease caused by Acinetobacter baumannii. Frontiers in Microbiology 2019;10:1601.
  3. Harding CM, Hennon SW, Feldman MF. Uncovering the mechanisms of Acinetobacter baumannii Nature Reviews Microbiology 2018;16(2):91-102.
  4. Shan W, Kan J, Cai X, Yin M. Insights into mucoid Acinetobacter baumannii: A review of microbiological characteristics, virulence, and pathogenic mechanisms in a threatening nosocomial pathogen. Microbiological Research 2022:127057.
  5. Monds RD, O’Toole GA. The developmental model of microbial biofilms: ten years of a paradigm up for review. Trends in Microbiology 2009;17(2):73-87.
  6. Stoodley P, Sauer K, Davies DG, Costerton JW. Biofilms as complex differentiated communities. Annual Reviews in Microbiology 2002;56(1):187-209.
  7. Beachey EH, Giampapa CS, Abraham SN. Bacterial adherence: adhesin receptor-mediated attachment of pathogenic bacteria to mucosal surfaces. American Review of Respiratory Disease 2012.
  8. Loehfelm TW, Luke NR, Campagnari AA. Identification and characterization of an Acinetobacter baumannii biofilm-associated protein. Journal of Bacteriology 2008;190(3):1036-44.
  9. Valle J, Latasa C, Gil C, Toledo-Arana A, Solano C, Penadés JR, et al. Bap, a Biofilm Matrix Protein of Staphylococcus aureus Prevents Cellular Internalization through Binding to GP96 Host Receptor. PLOS Pathogens 2012;8(8):e1002843.
  10. Rahbar MR, Rasooli I, Mousavi Gargari SL, Amani J, Fattahian Y. In silico analysis of antibody triggering biofilm associated protein in Acinetobacter baumannii. Journal of Theoretical Biology 2010;266(2):275-90.
  11. Crosa JH. Genetics and molecular biology of siderophore-mediated iron transport in bacteria. Microbiological Reviews 1989;53(4):517-30.
  12. Ratledge C, Dover LG. Iron metabolism in pathogenic bacteria. Annual Reviews in Microbiology 2000;54(1):881-941.
  13. Gaddy JA, Arivett BA, McConnell MJ, López-Rojas R, Pachón J, Actis LA. Role of acinetobactin-mediated iron acquisition functions in the interaction of Acinetobacter baumannii strain ATCC 19606T with human lung epithelial cells, Galleria mellonella caterpillars, and mice. Infection and Immunity 2012;80(3):1015-24.
  14. Eijkelkamp BA, Hassan KA, Paulsen IT, Brown MH. Investigation of the human pathogen Acinetobacter baumannii under iron limiting conditions. BMC Genomics 2011;12(1):126.
  15. Finne J, Leinonen M, Mäkelä PH. Antigenic similarities between brain components and bacteria causing meningitis: implications for vaccine development and pathogenesis. The Lancet 1983;322(8346):355-7.
  16. Weinberg ED. Iron withholding: a defense against infection and neoplasia. Physiological Reviews 1984;64(1):65-102.
  17. Esmaeilkhani H, Rasooli I, Nazarian S, Sefid F. In vivo validation of the immunogenicity of recombinant baumannii acinetobactin utilization a protein (rBauA). Microbial Pathogenesis 2016;98:77-81.
  18. Sefid F, Rasooli I, Jahangiri A. In silico determination and validation of baumannii acinetobactin utilization a structure and ligand binding site. BioMed Research International 2013. https://doi.org/10.1155/2013/172784.
  19. Sefid F, Rasooli I, Jahangiri A, Bazmara H. Functional exposed amino acids of BauA as potential immunogen against Acinetobacter baumannii. Acta Biotheoretica 2015;63(2):129-49.
  20. Rasooli I, Abdolhamidi R, Jahangiri A, Astaneh SDA. Outer membrane protein, Oma87 prevents Acinetobacter baumannii International Journal of Peptide Research and Therapeutics 2020;26(4):2653-60.
  21. Wu T, Malinverni J, Ruiz N, Kim S, Silhavy TJ, Kahne D. Identification of a multicomponent complex required for outer membrane biogenesis in Escherichia coli. Cell 2005;121(2):235-45.
  22. Sklar JG, Wu T, Gronenberg LS, Malinverni JC, Kahne D, Silhavy TJ. Lipoprotein SmpA is a component of the YaeT complex that assembles outer membrane proteins in Escherichia coli. Proceedings of the National Academy of Sciences 2007;104(15):6400-5.
  23. Bos MP, Robert V, Tommassen J. Functioning of outer membrane protein assembly factor Omp85 requires a single POTRA domain. EMBO Reports 2007;8(12): 1149-54.
  24. Singh R, Capalash N, Sharma P. Immunoprotective potential of BamA, the outer membrane protein assembly factor, against MDR Acinetobacter baumannii. Scientific Reports 2017;7(1):1-11.
  25. Singh R, Capalash N, Sharma P. Vaccine development to control the rising scourge of antibiotic-resistant Acinetobacter baumannii: a systematic review. Biotech 2022; 12(3):1-14.
  26. McConnell MJ, Martín-Galiano AJ. Designing multi-antigen vaccines against Acinetobacter baumannii using systemic approaches. Frontiers in Immunology 2021;12:666742.
  27. Xiong YQ, Estellés A, Li L, Abdelhady W, Gonzales R, Bayer AS, et al. A human biofilm-disrupting monoclonal antibody potentiates antibiotic efficacy in rodent models of both Staphylococcus aureus and Acinetobacter baumannii Antimicrobial Agents and Chemotherapy 2017; 61(10):e00904-17.
  28. Devi SS. Iron regulated outer membrane proteins (IROMPs) as potential targets against carbapenem-resistant Acinetobacter isolated from a Medical Centre in Malaysia. Indian Journal of Medical Research 2010; 131:578-83.
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