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Sheikhmohammadi, Matineh, Karami, Manizheh, Hajnorouzi, Abazar. (1401). Effectiveness of Fe2O3 nanoparticles more than magnetic field against the destructive effect of colchicine on Paramecium caudatum. , 10(1), 30-37. doi: 10.22070/jbcp.2022.16351.1158
Matineh Sheikhmohammadi; Manizheh Karami; Abazar Hajnorouzi. "Effectiveness of Fe2O3 nanoparticles more than magnetic field against the destructive effect of colchicine on Paramecium caudatum". , 10, 1, 1401, 30-37. doi: 10.22070/jbcp.2022.16351.1158
Sheikhmohammadi, Matineh, Karami, Manizheh, Hajnorouzi, Abazar. (1401). 'Effectiveness of Fe2O3 nanoparticles more than magnetic field against the destructive effect of colchicine on Paramecium caudatum', , 10(1), pp. 30-37. doi: 10.22070/jbcp.2022.16351.1158
Sheikhmohammadi, Matineh, Karami, Manizheh, Hajnorouzi, Abazar. Effectiveness of Fe2O3 nanoparticles more than magnetic field against the destructive effect of colchicine on Paramecium caudatum. , 1401; 10(1): 30-37. doi: 10.22070/jbcp.2022.16351.1158

Effectiveness of Fe2O3 nanoparticles more than magnetic field against the destructive effect of colchicine on Paramecium caudatum

Journal of Basic and Clinical Pathophysiology
دوره 10، شماره 1، شهریور 2022، صفحه 30-37    اصل مقاله (677.88 K)
نوع مقاله: Research Paper
شناسه دیجیتال (DOI): 10.22070/jbcp.2022.16351.1158
نویسندگان
Matineh Sheikhmohammadi1؛ Manizheh Karami* 1؛ Abazar Hajnorouzi2
1Department of Biology, Faculty of Basic Sciences, Shahed University, Tehran, Iran Iran
2Department of Physics, Faculty of Basic Sciences, Shahed University, Tehran, Iran
چکیده
Background and Objective: Colchicine depolymerizes microtubules and reduces their diamagnetic capacity. We investigated the effect of a static magnetic field (SMF) and the Fe2O3 NPs on the Paramecium caudatum exposed to colchicine.
Materials and Methods: The samples were collected from temporary water sources and after identifying the species, were cultured in the laboratory. They were also sub-cultured at regular intervals (7-10 days) for purification. From pure culture, several samples were placed in the laboratory or under SMF with an intensity of 61 mT for 3 days. To evaluate the effect of materials in comparison with distilled water (control), a sample (0.1 ml) of each medium was placed on a slide and exposed to 1 μl of distilled water or volume-concentrations of colchicine (0.05 to 25 μg/μl) or Fe2O3 NPs (0.05 to 3 μg/μl). The effect of different concentrations of Fe2O3 NPs in accompany with the field was also investigated. The movement of the animal was examined for 30 sec under a constant view and the sample was fixed for staining and study at the cellular level. Data were analyzed using analysis of variance.
Results: Colchicine at high concentrations (15 and 25 μg/μl) significantly reduced the motility of Paramecium caudatum. Magnetic field, but not NPs, alone reduced cell motility. Co-exposure to particles completely improved cell motility due to exposure to colchicine, however, in animals initially housed in the magnetic field, the healing effect of the particles was impaired.
Conclusion: The protective effects of NPs may depend on the diamagnetic capacity of the microtubule.
کلیدواژه‌ها
Paramecium caudatum؛ Colchicine؛ Static magnetic field؛ Fe2O3 nanoparticles
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