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Hajghasemi, Fatemeh, Khalili, Mohsen. (1395). Profile of interleukin-2 production in human mononuclear cells and T-cell lines. , 4(2), 5-10. doi: 10.22070/jbcp.2016.366
Fatemeh Hajghasemi; Mohsen Khalili. "Profile of interleukin-2 production in human mononuclear cells and T-cell lines". , 4, 2, 1395, 5-10. doi: 10.22070/jbcp.2016.366
Hajghasemi, Fatemeh, Khalili, Mohsen. (1395). 'Profile of interleukin-2 production in human mononuclear cells and T-cell lines', , 4(2), pp. 5-10. doi: 10.22070/jbcp.2016.366
Hajghasemi, Fatemeh, Khalili, Mohsen. Profile of interleukin-2 production in human mononuclear cells and T-cell lines. , 1395; 4(2): 5-10. doi: 10.22070/jbcp.2016.366

Profile of interleukin-2 production in human mononuclear cells and T-cell lines

Journal of Basic and Clinical Pathophysiology
مقاله 2، دوره 4، شماره 2، تیر 2016، صفحه 5-10    اصل مقاله (498.85 K)
نوع مقاله: Research Paper
شناسه دیجیتال (DOI): 10.22070/jbcp.2016.366
نویسندگان
Fatemeh Hajghasemi* 1؛ Mohsen Khalili2
1Department of Immunology, Faculty of Medicine, Shahed University, Tehran, Iran.
2Department of Physiology, Faculty of Medicine, Shahed University, Tehran, Iran.
چکیده
Introduction: Interleukin-2 (IL-2) as a T helper type 1 (Th1) cytokine has an important role Background and Objective: Interleukin-2 (IL-2) as a T helper type 1 (Th1) cytokine has an important role in activation, growth and differentiation of several immune cells. Moreover IL-2 is known as a pro-inflammatory and anti-tumoral cytokine. In addition deysregulation of IL-2 in some diseases such as autoimmunity has been shown. The present study was conducted to evaluate the patterns of IL-2 production in human peripheral blood mononuclear cells (PBMCs) and human leukemic T cells lines (Molt-4 and Jurkat).

Materials and Methods: Human PBMCs and leukemic T cells were cultured in complete RPMI-1640 medium. The cells were then seeded at a density of 106 cells/ml and were incubated with different concentrations of phorbol myristate acetate (PMA) (1-25 ng/ml) or phytoheamagglutinin (PHA) (2-10 μg/ml) for 48 hours. Then the cell-conditioned media were collected and used for IL-2 assay. Statistical comparisons between groups were made by analysis of variance (ANOVA).

Results: PHA/PMA significantly and dose-dependently increased IL-2 level in human PBMCs and leukemic T cells (Molt-4 and Jurkat) after 48 hours of incubation compared with untreated control cells.

Conclusion: We conclude that human PBMCs and leukemic T cells (Jurkat and Molt-4) could potentially secrete IL-2 with different degrees. Thus, these cells could offer a proper system to study the mechanisms regulating IL-2 production in diseases in which IL-2 production is disregulated. Also these cells could be useful for screening the IL-2 modulators.
کلیدواژه‌ها
IL-2؛ leukemic؛ T-cells؛ PBMCs
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