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Hoseinpour, Fatemeh, Hashemnia, Mohammad, Cheraghi, Hadi, Safikhani, Reza, Sheikhi, Tahereh, Hasani, Zahra. (1400). Assessment of antioxidant and cutaneous wound healing effects of Ornithogalum cuspidatum hydroalcoholic extract in male Wistar rats. , 9(1), 23-32. doi: 10.22070/jbcp.2021.14242.1140
Fatemeh Hoseinpour; Mohammad Hashemnia; Hadi Cheraghi; Reza Safikhani; Tahereh Sheikhi; Zahra Hasani. "Assessment of antioxidant and cutaneous wound healing effects of Ornithogalum cuspidatum hydroalcoholic extract in male Wistar rats". , 9, 1, 1400, 23-32. doi: 10.22070/jbcp.2021.14242.1140
Hoseinpour, Fatemeh, Hashemnia, Mohammad, Cheraghi, Hadi, Safikhani, Reza, Sheikhi, Tahereh, Hasani, Zahra. (1400). 'Assessment of antioxidant and cutaneous wound healing effects of Ornithogalum cuspidatum hydroalcoholic extract in male Wistar rats', , 9(1), pp. 23-32. doi: 10.22070/jbcp.2021.14242.1140
Hoseinpour, Fatemeh, Hashemnia, Mohammad, Cheraghi, Hadi, Safikhani, Reza, Sheikhi, Tahereh, Hasani, Zahra. Assessment of antioxidant and cutaneous wound healing effects of Ornithogalum cuspidatum hydroalcoholic extract in male Wistar rats. , 1400; 9(1): 23-32. doi: 10.22070/jbcp.2021.14242.1140

Assessment of antioxidant and cutaneous wound healing effects of Ornithogalum cuspidatum hydroalcoholic extract in male Wistar rats

Journal of Basic and Clinical Pathophysiology
مقاله 3، دوره 9، شماره 1، مرداد 2021، صفحه 23-32    اصل مقاله (790.53 K)
نوع مقاله: Research Paper
شناسه دیجیتال (DOI): 10.22070/jbcp.2021.14242.1140
نویسندگان
Fatemeh Hoseinpour* 1؛ Mohammad Hashemnia2؛ Hadi Cheraghi3؛ Reza Safikhani3؛ Tahereh Sheikhi3؛ Zahra Hasani3
1Department of Basic Sciences, Faculty of Veterinary Medicine, Razi University, Kermanshah, Iran
2Department of Pathobiology, Faculty of Veterinary Medicine, Razi University, Kermanshah, Iran
3Department of Clinical Sciences, Faculty of Veterinary Medicine, Razi University, Kermanshah, Iran.
چکیده
Background and Objective: Ornithogalum cuspidatum is a medicinal plant in Iranian traditional medicine that has several pharmacological effects. Due to strong antioxidant and anti-inflammatory activities of this plant, the current study was designed to evaluate wound healing activity of O. cuspidatum on cutaneous wounds in Wistar rats.
Materials and Methods: A full-thickness excisional wounds was induced on the back of 50 Wistar rats. The animals were randomly divided into five groups, including control, basal cream, phenytoin, O. cuspidatum 5%, and O. cuspidatum 10%. Five animals of each group were euthanized at 10 and 20 days post-injury (DPI) and wounds were assessed through gross and histopathological analyses. Also, hydroxyproline content and MDA, NO and TOS concentrations were determined.
Results: Treated animals with O. cuspidatum showed a significant reduction in the wound surface area at 10 and 20 dpi. Moreover, treatment with this plant reduced the number of lymphocytes and macrophages, increased the number of fibroblasts at the earlier stages and enhanced number of fibrocytes at the later stages of wound healing. O. cuspidatum significantly improved re-epithelialization and epithelial formation, enhanced hydroxyproline content and thereby maturity of the collagen fibers. Also, O. cuspidatum significantly reduced MDA, NO and TOS concentration as oxidant status in granulation tissue.
Conclusion: The present study demonstrated that application of hydroethanolic extract of O. cuspidatum promoted wound healing due to increased re-epithelialization and collagen deposition in wound tissue and also induction of considerable wound contraction, so it can be considered as a therapeutic agent for wound healing.
کلیدواژه‌ها
Ornithogalum cuspidatum؛ Antioxidant؛ Wound healing
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