Assessment of Antitumor Activity of Vinca herbacea on Human Ovarian Cancer Cell Line | ||
| Immunoregulation | ||
| دوره 3، شماره 2 - شماره پیاپی 6، فروردین 2020، صفحه 115-126 اصل مقاله (1.22 M) | ||
| نوع مقاله: Original Article | ||
| شناسه دیجیتال (DOI): 10.32598/Immunoregulation.3.2.6 | ||
| نویسندگان | ||
| Somayeh Dehghanipour* 1؛ Sara Saadatmand2؛ Nasim Hayati Roodbari3؛ Mehdi Mahdavi* 4 | ||
| 1Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran. | ||
| 2Recombinant Vaccine Research Center, Tehran University of Medical Sciences, Tehran, Iran. | ||
| 3Immunotherapy Group, Institute of Pharmaceutical Sciences, Tehran University of Medical Sciences, Tehran, Iran. | ||
| 4Department of Immunology, Pasteur Institute of Iran, Tehran, Iran. | ||
| چکیده | ||
| Background: It seems that Vinca. herbacea has an anti-tumor effect. Here, the immunotherapeutic effect of this compound is assessed against human ovarian cancer (SKOV3) cells because of the high incidence of this tumor in women. Materials and Methods: The cytotoxic activity of V. herbacea extract against human ovarian cancer (SKOV3) cells was determined by MTT assay. The apoptosis-inducing potential of V. herbacea extract was investigated using the FITC-V Annexin kit. The Matrigel invasion assay was used to investigate the ability of V. herbacea extract in reducing ovarian cancer cells invasion. Real-time PCR using specific primers was performed to investigate the expression of angiogenesis (VEGFR1, VEGFR2, and VEGF-A), apoptosis (Bcl-2 and Bax), and metastasis (MMP2 and MMP9) genes. Results: V. herbacea caused a significant cytotoxic effect against human ovarian cancer cells in a dose-dependent manner. V. herbacea induced apoptosis in SKOV3 cells through caspase-3 activation and an increase in the expression ratio of Bax/Bcl-2. V. herbacea inhibited cancer cells’ angiogenesis, which was evident by the significant reduction in the expression of angiogenesis-related genes, including VEGF, VEGFR-1, and VEGFR-2. Besides, V. herbacea inhibited cancer cell adhesion and invasion. Conclusion: V. herbacea extract elicits a robust cytostatic effect in SKOV3 cells by modulating the activity and or the expression of proteins regulating the process of cellular apoptosis, adhesion invasion, and angiogenesis. | ||
| کلیدواژهها | ||
| Vinca herbacea extract؛ Ovarian cancer؛ Angiogenesis؛ Apoptosis؛ Antitumor | ||
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آمار تعداد مشاهده مقاله: 361 تعداد دریافت فایل اصل مقاله: 378 |
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